Iowa State University NSF
Assembly Statistics
MAGIs 4.0
MAGIs 3.1
Input data (No. GSS)
3,126,130
879,523
Assembly Size (MB)
268.1
176.9
% GC
45.2
45.6
% bp Masked
50.4
14.7
No. Contigs
163,390
114,173
Avg No. GSS per Contig
6.55
5.85
Average Contig Length (bp)
1,641
1,550
Maximum Contig Length (bp)
36,517
12,498
No. Singletons
536,377
212,127


Repeat Masking
MAGIs 4.0
MAGIs 3.1
HC
3% of 357 MB
6.8% of 280 MB
MF
14% of 335 MB
15.9% of 266.2 MB
Shotgun
64% of 870 MB
74% of 31.7 MB
BAC
68% of 964 MB
-
Total
50.4% of 2526 MB
14.6% of 577.9 MB


Distribution of Contig Size in MAGIs 4.0 and MAGIs 3.1
Size
MAGIs 4.0

No. of Contigs Avg No. GSS per Contig

MAGIs 3.1

No. of Contigs Avg No. GSS per Contig

< 1 kb
33,969 2.28
32,034 2.31
1 ≤ size < 2kb
93,077 3.92
57,647 4.27
2 ≤ size < 3kb
24,007 10.21
16,088 10.61
3 ≤ size < 4kb
7,625 18.05
5,312 17.10
4 ≤ size < 5kb
2556 26.52
1946 23.56
5 ≤ size < 6kb
1011 40.23
694 30.19
6 ≤ size < 7kb
436 59.86
267 37.75
<7 ≤ size < 8kb
222 79.5
115 43.85
8 ≤ size < 9kb
117 118.38
43 49.33
9 ≤ size < 10kb
72 135.96
16 57.19
10 ≤ size < 11kb
58 174.52
8 67.88
11 ≤ size < 12kb
56 180.46
3 81.67
12 ≤ size < 13kb
44 188.59
1 72
13 ≤ size < 14kb
24 218.38
0 0
14 ≤ size < 15kb
19 259.74
0 0
> 15kb
97 318.47
0 0


Clone Statistics for MAGIs 3.1 and MAGI 4.0

Total Number of plasmid clones 512,484 710,114
MAGIs composed of a single clone 28,501 22,856
Clones assembled with other clones into MAGIs 389,799 687,336
Average number of clones per MAGI 3.66 4.98
Maximum number of clones per MAGI 124 740
Clones split between two assembled MAGIs 23,711 104,203


Click here to see the assembly methods.