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Marker Details
IMR8
IMR
Dominant
+/- (B73/Mo17)
Chr01 - 18.0 cM (View on CMap)
Sequences
MAGIv3.1_62261
MAGIv3.1_62261; MAGIv4_37970
76017308; 156623_3215_0893
Primer Info
Forward 31bMAGI_62261L3 GGGGAGAATAAGGGGACAAA 20
Reverse 31bMAGI_62261R3 TAGCGCTGTGCAGAGACTGT 20
Gradient Test of Primer
Setup Conditions for 20µl PCR Reaction PCR Program
DNA (~20 ng) 2.5 µl
10X PCR Buffer 2.0 µl
2 mM dNTP 2.0 µl
50 mM MgCl2 0.8 µl
5 µM Forward Primer 2.0 µl
5 µM Reverse Primer 2.0 µl
Taq (1u/µl) 1.0 µl
dH2O 7.7 µl
Total 20.0 µl
  • Step 1: 94°C - 3 mins
  • Step 2: 94°C - 30 secs
  • Step 3: Annealing Temp °C(Tm) - 45 secs
  • Step 4: 72°C - 1 min 30 secs
  • Step 5: Go to step 2 for 30 cycles
  • Step 6: 72°C - 10 mins
  • Step 7: END
Tm values were calculated by primer3 design software. Details on their implementation can be found here. For a complete list of Tm values for all ISU mapped markers, click here.
« Download IBM 91RI Scores »
Intermated B73 x Mo17 (IBM) Map
IBM Map Scores
1
M0001
1
2
M0005
2
3
M0007
1
4
M0008
2
5
M0010
1
6
M0012
1
7
M0014
1
8
M0015
1
9
M0016
1
10
M0017
1
11
M0021
2
12
M0022
1
13
M0023
2
14
M0024
1
15
M0025
2
16
M0027
2
17
M0028
1
18
M0029
2
19
M0030
2
20
M0031
1
21
M0032
2
22
M0033
2
23
M0034
2
24
M0035
1
25
M0039
1
26
M0040
2
27
M0043
2
28
M0045
1
29
M0046
2
30
M0048
2
31
M0051
2
32
M0052
1
33
M0054
2
34
M0055
1
35
M0057
1
36
M0058
2
37
M0060
1
38
M0061
1
39
M0063
1
40
M0066
1
41
M0067
2
42
M0074
2
43
M0075
1
44
M0076
1
45
M0077
2
46
M0079
1
47
M0262
1
48
M0264
2
49
M0265
1
50
M0266
1
51
M0267
1
52
M0269
2
53
M0272
1
54
M0275
2
55
M0276
1
56
M0281
2
57
M0284
2
58
M0287
1
59
M0288
2
60
M0296
2
61
M0297
2
62
M0298
2
63
M0309
2
64
M0310
2
65
M0311
2
66
M0315
2
67
M0317
2
68
M0321
2
69
M0322
1
70
M0323
2
71
M0325
1
72
M0326
1
73
M0328
1
74
M0337
2
75
M0341
1
76
M0344
1
77
M0345
1
78
M0346
2
79
M0352
1
80
M0354
1
81
M0355
2
82
M0357
1
83
M0360
2
84
M0364
2
85
M0365
2
86
M0368
2
87
M0369
1
88
M0378
2
89
M0379
1
90
M0382
2
91
M0384
2
          

IBM Map Legend
Missing Data or Heterozygous 0
B73 1
Mo17 2