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Iowa State University NSF
Marker Details
TIDP5514
TIDP
Co-Dominant
TGCE
Chr01 - 1.8 cM (View on CMap)
Sequences
32098368
MAGIv4_107762; 32098368
MEC_87711_P95-Mar06; 099564_1273_1286; 047405_2525_1936; 045049_0254_3810; 116460_0415_2164; 168907_0276_3788; 150978_1170_1516; 210868_3727_3262
Primer Info
Forward G32098368FE TCAAAGCCATGAAGAAAGGC 20
Reverse G32098368RE ACCTTCAGTGTCGTCTTGGG 20
Gradient Test of Primer
Setup Conditions for 20µl PCR Reaction PCR Program
DNA (~20 ng) 2.5 µl
10X PCR Buffer 2.0 µl
2 mM dNTP 2.0 µl
50 mM MgCl2 0.8 µl
5 µM Forward Primer 2.0 µl
5 µM Reverse Primer 2.0 µl
Taq (1u/µl) 1.0 µl
dH2O 7.7 µl
Total 20.0 µl
  • Step 1: 94°C - 3 mins
  • Step 2: 94°C - 30 secs
  • Step 3: Annealing Temp °C(Tm) - 45 secs
  • Step 4: 72°C - 1 min 30 secs
  • Step 5: Go to step 2 for 30 cycles
  • Step 6: 72°C - 10 mins
  • Step 7: END
Tm values were calculated by primer3 design software. Details on their implementation can be found here. For a complete list of Tm values for all ISU mapped markers, click here.
« Download Inbred Scores »
24-Inbred Survey
  • Setup conditions for 20µl PCR reaction: same as described above, use 60°C as Annealing Temperature.
Inbred Scores
1
B109
1
2
Pa91
1
3
Va35
1
4
B84
1
5
N194
1
6
H84
1
7
N801W
1
8
N209
1
9
B113
1
10
B77
1
11
NC314
1
12
Mo44
1
13
B104
1
14
NC264
1
15
B73
1
16
Mo17
1
17
N7A
1
18
Oh43
1
19
R177
1
20
N28Ht
1
21
N215
1
22
C123
1
23
SD46
1
24
Va85
1

Score Legend
+ + + B=M=I 1
+ + + B=M, B≠I, M≠I 2
+ + + B≠M≠I 3
+ + + B≠M, B=I, M≠I 4
+ + + B≠M, B≠I, M=I 5
+ + - B=M 6
+ + - B≠M 7
+ - -   8
+ - + B=I 9
+ - + B≠I 10
- + -   11
- + + M=I 12
- + + M≠I 13
- - -   14
- - +   15
« Download IBM 91RI Scores »
Intermated B73 x Mo17 (IBM) Map
IBM Map Scores
1
M0001
2
2
M0005
2
3
M0007
1
4
M0008
2
5
M0010
2
6
M0012
2
7
M0014
1
8
M0015
1
9
M0016
1
10
M0017
1
11
M0021
1
12
M0022
2
13
M0023
2
14
M0024
2
15
M0025
1
16
M0027
1
17
M0028
1
18
M0029
1
19
M0030
1
20
M0031
1
21
M0032
2
22
M0033
1
23
M0034
1
24
M0035
1
25
M0039
1
26
M0040
2
27
M0043
2
28
M0045
2
29
M0046
2
30
M0048
2
31
M0051
1
32
M0052
1
33
M0054
2
34
M0055
2
35
M0057
2
36
M0058
2
37
M0060
2
38
M0061
1
39
M0063
1
40
M0066
2
41
M0067
2
42
M0074
1
43
M0075
1
44
M0076
1
45
M0077
2
46
M0079
1
47
M0262
2
48
M0264
1
49
M0265
1
50
M0266
1
51
M0267
1
52
M0269
2
53
M0272
2
54
M0275
2
55
M0276
1
56
M0281
2
57
M0284
2
58
M0287
1
59
M0288
1
60
M0296
1
61
M0297
2
62
M0298
2
63
M0309
2
64
M0310
2
65
M0311
1
66
M0315
2
67
M0317
1
68
M0321
1
69
M0322
1
70
M0323
2
71
M0325
1
72
M0326
2
73
M0328
1
74
M0337
2
75
M0341
1
76
M0344
1
77
M0345
2
78
M0346
1
79
M0352
1
80
M0354
1
81
M0355
2
82
M0357
2
83
M0360
1
84
M0364
1
85
M0365
1
86
M0368
2
87
M0369
1
88
M0378
2
89
M0379
1
90
M0382
1
91
M0384
2
          

IBM Map Legend
Missing Data or Heterozygous 0
B73 1
Mo17 2